Journal: Frontiers in Pharmacology

Article Title: Intermittent Fasting Inhibits High-Fat Diet–Induced Atherosclerosis by Ameliorating Hypercholesterolemia and Reducing Monocyte Chemoattraction

doi: 10.3389/fphar.2021.719750

Figure Lengend Snippet: Sequences of primers for qRT-PCR.

Article Snippet: Mouse anti–smooth muscle α-actin (SMA) monoclonal antibody and rabbit anti-collagen type III alpha 1 (COL3A1) polyclonal antibody were purchased from Affinity Biosciences (Cincinnati, OH, United States).

Techniques:

Journal: Frontiers in Pharmacology

Article Title: Intermittent Fasting Inhibits High-Fat Diet–Induced Atherosclerosis by Ameliorating Hypercholesterolemia and Reducing Monocyte Chemoattraction

doi: 10.3389/fphar.2021.719750

Figure Lengend Snippet: Intermittent fasting increases collagen content via inducing COL1A1 and COL3A1 expression. (A,B) The aortic root cross sections were used to determine collagen content by Picrosirius red staining with quantitative analysis of collagen positive areas. * p < 0.05 ( n = 6). (C,D) The expression of COL1A1, COL3A1, MMP2, and MMP9 in plaques were determined by immunofluorescent staining with the quantification of positive staining areas. * p < 0.05, ns: not significant ( n = 6). E : LDLR−/− mice were divided into the control or IF group ( n = 6/group). After 2 weeks HFD feeding ad libitum or IF treatment, the mice aorta samples were collected. The expression of COL1A1, COL3A1, MMP2, and MMP9 were determined by qRT-PCR. * p < 0.05, ns: not significant ( n = 6).

Article Snippet: Mouse anti–smooth muscle α-actin (SMA) monoclonal antibody and rabbit anti-collagen type III alpha 1 (COL3A1) polyclonal antibody were purchased from Affinity Biosciences (Cincinnati, OH, United States).

Techniques: Expressing, Staining, Control, Quantitative RT-PCR